Spermine isolated and identified as the major trypanocidal compound from the snake venom of Eristocophis macmahoni causes autophagy in Trypanosoma brucei.
Toxicon
; 50(4): 457-69, 2007 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-17548100
ABSTRACT
The snake venom from the leaf-nosed viper Eristocophis macmahoni was analyzed regarding its toxic effects on the bloodstream form of Trypanosoma brucei. A considerable trypanocidal effect was measured with an IC5 value of 186 ng/ml in bloodstream form parasites. Following several high performance liquid chromatography (HPLC) separation steps, the major trypanocidal activity was assigned to a single fraction by in vitro toxicity assays. Analysis by off-line ESI-MS(n) revealed an m/z value of 202.2 for the precursor ion and fragment ions of m/z=129.1 (MS2) and 112.1 (MS3), respectively, clearly corresponding to the molecular mass and the fragmentation pattern of the polyamine spermine. Quantification of spermine within the viper venom using an on-line hydrophilic interaction chromatography (HILIC) ESI-MS method revealed that this compound constituted approximately 1% of the dry venom mass. The polyamine oxidase activity in the fetal calf serum used for cultivation was responsible for a trypanocidal effect of pure spermine in the low micromolar range, whereas the antitrypanosomal activity of crude snake venom was virtually independent from serum, suggesting the oxidation of spermine by intrinsic venom components. Using fetal calf serum, spermine was shown to induce autophagy in the parasites using transmission electron microscopy (TEM).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Tripanossomicidas
/
Trypanosoma brucei brucei
/
Venenos de Víboras
/
Espermina
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Alemanha