Comparison of gene targeting efficiencies in two mosses suggests that it is a conserved feature of Bryophyte transformation.
Biotechnol Lett
; 29(10): 1591-8, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-17565445
ABSTRACT
The moss, Physcomitrella patens, is a novel tool in plant functional genomics due to its exceptionally high gene targeting efficiency that is so far unique for plants. To determine if this high gene targeting efficiency is exclusive to P. patens or if it is a common feature to mosses, we estimated gene-targeting efficiency in another moss, Ceratodon purpureus. We transformed both mosses with replacement vectors corresponding to the adenine phosphoribosyl transferase (APT) reporter gene. We achieved a gene targeting efficiency of 20.8% for P. patens and 1.05% for C. purpureus. Our findings support the hypothesis that efficient gene targeting could be a general mechanism of Bryophyte transformation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transformação Genética
/
Marcação de Genes
/
Briófitas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
França