Trimeric G-proteins of the trans-Golgi network are involved in the formation of constitutive secretory vesicles and immature secretory granules.
FEBS Lett
; 294(3): 239-43, 1991 Dec 09.
Article
em En
| MEDLINE
| ID: mdl-1756866
ABSTRACT
Non-hydrolysable analogues of GTP, such as GTP gamma S and GMP-PNP, have previously been shown to inhibit the formation of constitutive secretory vesicles (CSVs) and immature secretory granules (ISGs) from the trans-Golgi network (TGN). Using a cell-free system, we show here that the formation of these vesicles is also inhibited by [A1F4]-, a compound known to act on trimeric G-proteins. Addition of highly purified G-protein beta gamma subunits stimulated, in a differential manner, the cell-free formation of both CSVs and ISGs. ADP-ribosylation experiments revealed the presence of a pertussis toxin-sensitive G-protein alpha subunit in the TGN. We conclude that trimeric G-proteins regulate the formation of secretory vesicles from the TGN.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos de Alumínio
/
Proteínas de Ligação ao GTP
/
Grânulos Citoplasmáticos
/
Complexo de Golgi
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Alemanha