Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids.
Genome
; 50(5): 443-50, 2007 May.
Article
em En
| MEDLINE
| ID: mdl-17612613
ABSTRACT
Somatic hybrids between the wild incongruent species Solanum bulbocastanum (2n = 2x = 24) and S. tuberosum haploids (2n = 2x = 24) have been characterized for their nuclear and cytoplasmic genome composition. Cytologic observations revealed the recovery of 8 (near-)tetraploid and 3 hexaploid somatic hybrids. Multicolor genomic in situ hybridization (GISH) analysis was carried out to study the genomic dosage of the parental species in 5 somatic hybrids with different ploidy. The GISH procedure used was effective in discriminating parental genomes in the hybrids; most chromosomes were unambiguously colored. Two (near-)tetraploid somatic hybrids showed the expected 22 cultivated-to-wild genomic dosage; 2 hexaploids revealed a 42 cultivated-to-wild genomic dosage, and 1 hexaploid had a 24 cultivated-to-wild genomic dosage. Characterization of hybrid cytoplasmic genomes was performed using gene-specific primers that detected polymorphisms between the fusion parents in the intergenic regions. The analysis showed that most of the somatic hybrids inherited the plastidial and mitochondrial DNA of the cultivated parent. A few hybrids, with a rearranged mitochondrial genome (showing fragments derived from both parents), were also identified. These results confirmed the potential of somatic hybridization in producing new variability for genetic studies and breeding.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quimera
/
Genoma de Planta
/
Solanum
Idioma:
En
Revista:
Genome
Assunto da revista:
GENETICA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Itália