Efficiency of short-term storage of equine semen in a simple-design cooling system.

Five experiments tested the efficiency of a simple, low-cost system (CP) for cooling and storing equine semen at 2.0 degrees C for 24 h and 48 h. Pantaneiro stallions of known fertility were used. Semen quality was evaluated for progressive motility (PM), plasma membrane integrity (PMI), and pregnancy rate. Experiment 1 showed that PM and PMI were similar between CP and the control (Equitainer) in cooled semen. In Experiment 2, the influence was evaluated of combinations (four treatments) of two volumes (50/100 ml) and two sperm concentrations (500/750x10(6)) on sperm quality of semen cooled and preserved by CP (cooling system replaced at 24 h). While PM decreased gradually from before cooling to 24 h and 48 h, PMI decreased only at the least and greatest sperm volume and concentrations. Storage time did not affect PMI. Results from Experiment 3 showed that CP maintained semen PM>or=30% in all samples 24 h after cooling and decreased to about 70% 42 h after cooling. Results from Experiments 4 and 5 confirmed semen quality after cooling and storage (24 h and 48 h, respectively), achieving a 69% pregnancy rate in the first estrous cycle when insemination occurred. Thus, the CP system is satisfactory for cooling and preserving equine semen for up to 48 h.