3H-serotonin as a marker of oscillatory insulin secretion in clonal beta-cells (INS-1).
FEBS Lett
; 581(21): 4080-4, 2007 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-17686476
ABSTRACT
Serotonin release from preloaded pancreatic beta-cells has been used as a marker for insulin release in studying exocytosis from single cells using the amperometric technique. We found that single pancreatic beta-cells exhibited oscillations in exocytosis with a period of 1-1.5 min as measured amperometrically by serotonin release. We also show that 3H-serotonin can be used to monitor exocytosis from intact and streptolysin-O permeabilized clonal insulin-secreting cells preloaded with labeled serotonin and that serotonin release correlated with insulin secretion in the same cells. The use of 3H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Relógios Biológicos
/
Serotonina
/
Células Secretoras de Insulina
/
Exocitose
/
Insulina
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Suécia