Validated liquid chromatographic method for quantitative determination of allicin in garlic powder and tablets.
J Sep Sci
; 30(16): 2703-7, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17763522
ABSTRACT
In the present study, an RP high performance liquid chromatographic method was developed and validated for the determination of allicin in garlic powder and tablets. Chromatographic separation was carried out on an RP-18(e )column (125 mm x 4 mm), using a mobile phase, consisting of methanol-water (5050 v/v), at a flow rate of 0.5 mL/min and UV detection at 220 nm. Ethylparaben was used as the internal standard. The assay was linear for allicin concentrations of 5.0-60.0 microg/mL. The RSD for precision was <6.14%. The accuracy was above 89.11%. The detection and quantification limits were 0.27 and 0.81 microg/mL, respectively. This method was used to quantify allicin in garlic powder samples. The results showed that the method described here is useful for the determination of allicin in garlic powder and tablets.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pós
/
Ácidos Sulfínicos
/
Comprimidos
/
Cromatografia Líquida
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Chile