[Cloning and characterization of a novel glutathione transferase gene from Penicillium chrysogenum].
Sheng Wu Gong Cheng Xue Bao
; 23(4): 618-22, 2007 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-17822032
ABSTRACT
Glutathione transferases (GSTs) are a family of multifunctional proteins that mainly catalyze the conjugation of intracellular glutathione (GSH) to a wide variety of endogenous and exogenous electrophilic compounds. GSTs play important roles in stress tolerance and in the detoxification metabolism in organisms. A novel GST gene, Pc gstB, was cloned from penicillin producing fungus Penicillium chrysogenum using RT-PCR. The open reading frame (ORF) of Pc gstB was 651 bp and encoded a peptide of 216 residues. The deduced amino acids sequence had conserved GST domain and showed 65% identity to the characterized Aspergillus fumigutus gstB. The entire ORF of Pc gstB was inserted into vector pTrc99A and transformed into Escherichia coli DH5alpha. Recombinant PcGstB was overexpressed and its GST activity toward substrate 1-chloro-2,4-dinitrobenzene (CDNB) was validated.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Penicillium chrysogenum
/
Proteínas Recombinantes
/
Proteínas Fúngicas
/
Glutationa Transferase
Idioma:
Zh
Revista:
Sheng Wu Gong Cheng Xue Bao
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China