Combined estrogen and ghrelin administration restores number of blood vessels and collagen type I/III ratio in the urethral and anal canal submucosa of old ovariectomized rats.
Int Urogynecol J Pelvic Floor Dysfunct
; 19(4): 547-52, 2008 Apr.
Article
em En
| MEDLINE
| ID: mdl-17876488
ABSTRACT
We compare the effects of estrogen and/or ghrelin on vascular counts and collagen I/III ratio of urethral and anal canal submucosa in old vs young-adult ovariectomized rats. Ovariectomized Fisher 344 rats (18 and 3 months old, n = 24 x 2) received 42 daily intraperitoneal 17-ss estradiol (10 microg/kg), ghrelin (2 microg/kg), both, or vehicle (n = 6 x 4 per group). Blood vessel counts and collagen I/III ratio were measured, respectively, by light microscopy and Western blot analysis with immunohistochemistry of ghrelin receptors. Estrogen significantly increased urethral and anal vascular counts and collagen I/III ratio in young-adult rats. In old rats, only combined estrogen/ghrelin administration significantly increased both variables. This was not observed with estrogen or ghrelin separately. Ghrelin receptors were immunostained in urethral and anal submucosa of all samples. Combined estrogen/ghrelin administration restored postovariectomy urethral and anal canal submucosal vessel number and collagen I/III ratio in old rats suggesting independent ageing effect.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canal Anal
/
Uretra
/
Ovariectomia
/
Neovascularização Fisiológica
/
Colágeno Tipo I
/
Colágeno Tipo III
/
Estradiol
/
Grelina
Limite:
Animals
Idioma:
En
Revista:
Int Urogynecol J Pelvic Floor Dysfunct
Assunto da revista:
GINECOLOGIA
/
UROLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Emirados Árabes Unidos