[Fluid shear stress increases the Ca2+ concentration in bone-marrow derived osteoclast-like cells].
Hua Xi Kou Qiang Yi Xue Za Zhi
; 25(4): 412-4, 2007 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-17896506
ABSTRACT
OBJECTIVE:
To study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress.METHODS:
Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software.RESULTS:
At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.CONCLUSION:
The Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoclastos
/
Medula Óssea
Idioma:
Zh
Revista:
Hua Xi Kou Qiang Yi Xue Za Zhi
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China