Breakpoint characterization: a new approach for segregation analysis of paracentric inversion in human sperm.
Mol Hum Reprod
; 13(10): 751-6, 2007 Oct.
Article
em En
| MEDLINE
| ID: mdl-17913851
ABSTRACT
Paracentric inversions (PAI) are structural chromosomal rearrangements generally considered to be harmless. Nevertheless, cases of viable recombinants have been reported, indicating the interest of studying the meiotic behaviour of these chromosomal abnormalities. To date, the few studies reported have been performed using either the human-hamster fertilization system or fluorescence in situ hybridization with centromeric or telomeric DNA probes. In order to improve the assessment of meiotic segregation in PAI, we present a new strategy based on the use of bacterial artificial chromosome (BAC) probes which allow a precise localization of chromosome breakpoints and the identification of all meiotic products in human sperm. Sperm samples from carriers of an inv(5) and an inv(14) were used to test this new high-resolution procedure.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Quebra Cromossômica
/
Inversão Cromossômica
Limite:
Humans
/
Male
Idioma:
En
Revista:
Mol Hum Reprod
Assunto da revista:
BIOLOGIA MOLECULAR
/
MEDICINA REPRODUTIVA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
França