Hybrid silicon/silicone (polydimethylsiloxane) microsystem for cell culture.
Conf Proc IEEE Eng Med Biol Soc
; 2006: 2490-3, 2006.
Article
em En
| MEDLINE
| ID: mdl-17946517
ABSTRACT
We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture and incubation. The micro-incubator is engineered through the integration of a silicon CMOS die for the heater and temperature sensor, with multilayer silicone PDMS (polydimethylsiloxane) structures namely, fluidic channels and a 4 mm diameter, 30 microL, culture well. A 25 micron thick PDMS membrane covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The packaging for the microsystem includes a flexible polyimide electronic ribbon cable and four fluidic ports that provide external interfaces to electrical energy, closed loop sensing and electronic control as well as solid and liquid supplies. The complete structure has a size of (2.5x2.5x0.6 cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Silício
/
Materiais Biocompatíveis
/
Técnicas de Cultura de Células
/
Engenharia Tecidual
/
Técnicas Analíticas Microfluídicas
/
Dimetilpolisiloxanos
/
Citometria de Fluxo
/
Nylons
Idioma:
En
Revista:
Conf Proc IEEE Eng Med Biol Soc
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos