Selenomethionine labeling of recombinant proteins.
Methods Mol Biol
; 389: 165-74, 2007.
Article
em En
| MEDLINE
| ID: mdl-17951642
ABSTRACT
Selenomethionine incorporation is a standard method for determining the phases in protein crystallography by single- or multiwavelength anomalous dispersion. Recombinant expression of selenomethionine-containing protein in non-auxotrophic Pichia pastoris strains yield an incorporation of about 50%. The expression of a mutated variant of Penicillium minioluteum dextranase in P. pastoris is used to illustrate the method utilized to obtain selenomethionyl-substituted protein and to show the phasing power of the acquired anomalous signal. The dextranase structure was solved using the anomalous signal achieved from 50% selenomethionine incorporation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Selenometionina
/
Coloração e Rotulagem
/
Proteínas Recombinantes
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Suécia