Proposed structural models of human factor Va and prothrombinase.
J Thromb Haemost
; 6(1): 83-9, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-17973648
ABSTRACT
BACKGROUND:
The prothrombinase complex consists of factor Xa, FVa, calcium ions, and phospholipid membrane. The prothrombinase complex plays a key role in the blood coagulation process.OBJECTIVE:
To derive solvent-equilibrated models of human FVa and the prothrombinase complex.METHODS:
Several modeling techniques have been employed, including homology modeling, protein-protein docking, and molecular dynamics simulation methods, to build the structural models. RESULTS ANDCONCLUSIONS:
We found, upon simulation, a possibly significant shift towards planarity of the five FVa domains. To estimate a prothrombinase structure, we docked an FXa model to the equilibrated FVa model using experimental data as docking filters. We found that simulation of the docked complex led to some changes in the protein-protein contacts, but not buried surface area, as compared to the initial docking model. Possible locations of prothrombin binding to prothrombinase are indicated.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tromboplastina
/
Modelos Moleculares
/
Fator Va
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Thromb Haemost
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos