Intracellular Ca2+ regulates free-running circadian clock oscillation in vivo.
J Neurosci
; 27(46): 12489-99, 2007 Nov 14.
Article
em En
| MEDLINE
| ID: mdl-18003827
ABSTRACT
Although circadian oscillation in dynamics of intracellular Ca2+ signals has been observed in both plant and animal cells, it has remained unknown whether Ca2+ signals play an in vivo role in cellular oscillation itself. To address this question, we modified the dynamics of intracellular Ca2+ signals in circadian pacemaker neurons in vivo by targeted expression of varying doses of a Ca2+ buffer protein in transgenic Drosophila melanogaster. Intracellular Ca2+ buffering in pacemaker neurons results in dose-dependent slowing of free-running behavioral rhythms, with average period >3 h longer than control at the highest dose. The rhythmic nuclear accumulation of a transcription factor known to be essential for cellular circadian oscillation is also slowed. We also determined that Ca2+ buffering interacts synergistically with genetic manipulations that interfere with either calmodulin or calmodulin-dependent protein kinase II function. These results suggest a role for intracellular Ca2+ signaling in regulating intrinsic cellular oscillation in vivo.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Parvalbuminas
/
Relógios Biológicos
/
Cálcio
/
Ritmo Circadiano
/
Sinalização do Cálcio
/
Drosophila melanogaster
Limite:
Animals
Idioma:
En
Revista:
J Neurosci
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos