Retinal flow cytometer.
Opt Lett
; 32(23): 3450-2, 2007 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-18059963
ABSTRACT
The in vivo flow cytometer is an instrument capable of continuous, real-time monitoring of fluorescently labeled cells in the circulation without the need to draw blood samples. However, the original system probes a single vessel in the mouse ear; the small sample volume limits the sensitivity of the technique. We describe an in vivo retinal flow cytometer that simultaneously probes five artery-vein pairs in the mouse eye by circularly scanning a small laser spot rapidly around the optic nerve head. We demonstrate that the retinal flow cytometer detects about five times more cells per minute than the original in vivo flow cytometer does in the ear.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasos Retinianos
/
Citometria de Fluxo
Limite:
Animals
Idioma:
En
Revista:
Opt Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos