Measurement of bone blood flow using the hydrogen washout Technique-Part I: quantitative evaluation of tissue perfusion in the laboratory rat.
J Orthop Res
; 26(6): 741-5, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18186132
ABSTRACT
The measurement of blood flow in bone is of interest in both clinical and experimental settings. Such determinations would ideally provide accurate, quantitative, and reproducible data with relatively simple and safe technology, even in the small bones of experimental animals. Methods that provide absolute or quantitative measurements include positron emission tomography, "isotope fractioning" using radioactive or fluorescent-labeled microspheres, and measurement of the rate of washout of diffusible tracers delivered either by injection or inhalation. In this article, we describe in detail a modification of the original Whiteside hydrogen washout technique, using modern hydrogen sensors, a micromanipulator for probe placement, and custom software that greatly simplifies blood flow determination and is effective in the small bones of experimental animals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fluxo Sanguíneo Regional
/
Osso e Ossos
/
Hidrogênio
/
Micromanipulação
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Orthop Res
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos