Alterations of the glycan moiety of human alpha 1-acid glycoprotein in late-term pregnancy.
Clin Chim Acta
; 204(1-3): 1-12, 1991 Dec 31.
Article
em En
| MEDLINE
| ID: mdl-1819453
ABSTRACT
The carbohydrate moiety of purified alpha 1-acid glycoprotein (AGP) from healthy male adults (AGPn) and late-term pregnant women (AGPp) was analysed. Polyacrylamide gel electrophoresis with sodium dodecyl sulfate before and after N-glycanase treatment showed that AGPp had a slightly higher molecular mass due to an enriched carbohydrate moiety. BIO-GEL P-4 and Concanavalin A (Con A)-Sepharose chromatography of the oligosaccharides released by hydrazinolysis and fractionated by high-voltage electrophoresis indicated a progression towards Con A-unbound oligosaccharides and towards larger glycans in pregnancy. Carbohydrate analysis of purified AGPp and AGPn and of the most increased oligosaccharide fraction (F4A) evidenced a decrease in the fucosyl molar ratio and a slight increase in the galactosyl, N-acetyl-glucosaminyl and N-acetyl neuraminyl ratios. These results suggest that AGP contains more highly branched oligosaccharides and/or additional N-acetyllactosamine-type oligosaccharides in pregnancy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Orosomucoide
/
Gravidez
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
França