Point mutation detection by surface plasmon resonance imaging coupled with a temperature scan method in a model system.
Anal Chem
; 80(4): 1049-57, 2008 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-18211033
ABSTRACT
The detection of point mutations in genes presents clear biological and medical interest. Various methods have been considered. In this paper, we take advantage of surface plasmon resonance imaging, a technique allowing detection of unlabeled DNA hybridization. Coupled with a temperature scan, this approach allows us to determine the presence of single-point mutations in oligonucleotide samples from the analysis of DNA's melting curves in either the homozygous or heterozygous case. Moreover, these experimental data are confirmed in good agreement with numerical calculations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Técnicas Biossensoriais
/
Mutação Puntual
/
Hibridização In Situ
/
Ressonância de Plasmônio de Superfície
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
França