In vitro effects of hydrochloric and lactic acids on bioelectric properties of equine gastric squamous mucosa.
Equine Vet J
; 40(4): 301-5, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18321809
ABSTRACT
REASONS FOR PERFORMING STUDY Volatile fatty acids, byproducts of carbohydrate fermentation by resident bacteria, have been implicated in causing nonglandular (NG) gastric ulcers. Lactic acid (LA), also produced by stomach bacteria, may cause gastric ulcers when exposed to the equine NG mucosa. OBJECTIVES:
To investigate the in vitro effects of LA on equine NG mucosa bioelectric properties, sodium transport and tissue resistance.METHODS:
Gastric tissues obtained from 13 mature horses were studied in Ussing chambers. Short-circuit current (Isc) and potential difference (PD) were measured, and electrical resistance (R) and conductance (G) calculated for tissues after addition of HCl and LA (5, 10, 20 and 40 mmol/l) in normal Ringer's solution (NRS).RESULTS:
Mucosa exposed to HCl or LA (5, 10 and 20 mmol/l) in NRS (pH 1.5 and to a lesser extent pH 4.0) had a significant decrease in Isc and PD. Mucosa exposed to a high concentration of LA (40 mmol/l) in NRS (LRS) at pH 1.5 showed an increased G, but this increase was not significant. Values returned to baseline after solutions were returned to pH 7.0. Histological changes were consistent with HCl-induced (pH <4.0) acid damage.CONCLUSIONS:
HCl induced alteration in bioelectric properties of equine NG mucosa whereas addition of LRS did not, other than those changes seen with HCl alone.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sódio
/
Ácido Láctico
/
Mucosa Gástrica
/
Ácido Clorídrico
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Equine Vet J
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos