Characterization of human mesothelioma cell lines as tumor models for suicide gene therapy.
Onkologie
; 31(3): 91-6, 2008 Mar.
Article
em En
| MEDLINE
| ID: mdl-18322411
ABSTRACT
BACKGROUND:
The median survival time of patients with malignant pleural mesothelioma (MPM) remains poor. Therefore, novel therapeutic options are in high demand, and well characterized model systems for in vitro/vivo screening have to be established. MATERIAL ANDMETHODS:
For this purpose, 3 MPM cell lines (H-Meso-1, MSTO211H, and NCI-H28) were characterized and tested for susceptibility to recombinant adeno-associated virus 2 (rAAV2)-based vectors which have the potential for a loco-regional application.RESULTS:
Using multiplex fluorescence in situ hybridization, several recurrent chromosomal aberrations were observed for each of the MPM cell lines. Tumorigenicity of H-Meso-1 and MSTO-211H cells was shown in an intraperitoneal NOD/SCID mouse model, whereas NCI-H28 cells did not yield any tumors. Although all 3 cell lines were readily susceptible to rAAV2 vectors, differences in susceptibility were observed (H-Meso-1 > NCI-H28 > MSTO-211H). Furthermore, the efficacy of a potential suicide gene therapy using an rAAV2 suicide vector-transduced MPM cell line was determined in a proof-of-feasibility in vivo experiment.CONCLUSION:
The characterized cell lines described here may serve as a model for in vitro and in vivo preclinical gene therapy for the treatment of MPM using rAAV2 suicide vectors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução Genética
/
Terapia Genética
/
Genes Transgênicos Suicidas
/
Mesotelioma
Limite:
Animals
Idioma:
En
Revista:
Onkologie
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Alemanha