High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol.
Comb Chem High Throughput Screen
; 11(2): 159-72, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-18336209
ABSTRACT
Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or--by using the recently developed RNA interference (RNAi) techniques--knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Análise Serial de Tecidos
/
Transcrição Reversa
/
Avaliação Pré-Clínica de Medicamentos
/
Genes
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Comb Chem High Throughput Screen
Assunto da revista:
BIOLOGIA MOLECULAR
/
QUIMICA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Alemanha