First evidence of a functional interaction between DNA quadruplexes and poly(ADP-ribose) polymerase-1.
ACS Chem Biol
; 3(4): 214-9, 2008 Apr 18.
Article
em En
| MEDLINE
| ID: mdl-18338862
ABSTRACT
We discovered that the abundant human nuclear protein poly(ADP-ribose) polymerase-1 (hPARP-1) binds to intramolecular DNA quadruplexes in vitro with high affinity and with a stoichiometry of two proteins for one quadruplex. Using an enzymatic assay, we have shown that hPARP-1 gets catalytically activated upon binding to G-quadruplexes localized at the c-kit promoter and human telomere regions. This is the first example of a truly functional quadruplex-protein interaction, which has possible implications in understanding hPARP-1 mediated mechanisms of transcription regulation and telomere end protection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli(ADP-Ribose) Polimerases
/
Quadruplex G
Limite:
Humans
Idioma:
En
Revista:
ACS Chem Biol
Ano de publicação:
2008
Tipo de documento:
Article