Determination of polyphenol components in herbal medicines by micellar electrokinetic capillary chromatography with Tween 20.
Talanta
; 74(1): 1-6, 2007 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-18371605
ABSTRACT
A simple and convenient method of micellar electrokinetic capillary chromatography (MEKC) using polyoxyethylene sorbitan monolaurate (Tween 20) to form single micelle and methanol as a buffer additive was introduced for the simultaneous determination of five polyphenols, including scopoletin, rutin, esculetin, chlorogenic acid and caffeic acid. A running buffer solution of pH 9.3, 20 mmol/L sodium tetraborate containing 64 mmol/L Tween 20 and 9% (v/v) methanol was adopted in the separation. Because rutin and esculetin were difficult to be separated by capillary zone electrophoresis (CZE) and SDS-based MEKC, Tween 20-based MEKC was adopted and the polyphenols were separated satisfactorily. The proposed method was used to determine the polyphenol components in the herbal medicine of Cortex fraxini. The separation mechanism of Tween 20-based MEKC for the polyphenols was discussed preliminarily.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenóis
/
Polissorbatos
/
Tensoativos
/
Flavonoides
/
Medicamentos de Ervas Chinesas
Idioma:
En
Revista:
Talanta
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China