A new method to measure cellular toxicity of non-fibrillar and fibrillar Alzheimer's Abeta using yeast.
J Alzheimers Dis
; 13(2): 147-50, 2008 Mar.
Article
em En
| MEDLINE
| ID: mdl-18376056
ABSTRACT
The 42 amino acid amyloid-beta (Abeta) can exist in multiple physical states including oligomers and fibrils. This study shows that fibril formation is hastened by the biological buffers required to support the growth of mammalian cells, but is prevented if Abeta is maintained in water. Here we describe a method to produce Abeta in oligomeric form and the comparison of stable fibrillar and non-fibrillar forms in cell toxicity studies in water, achieved through the use of yeast. We show that extracellular, non-fibrillar Abeta causes a dose dependent loss of cell viability while fibrillar Abeta has low toxicity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Peptídeos beta-Amiloides
/
Candida glabrata
/
Doença de Alzheimer
/
Amiloide
Limite:
Aged
/
Humans
Idioma:
En
Revista:
J Alzheimers Dis
Assunto da revista:
GERIATRIA
/
NEUROLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Austrália