Synthesis of covalent oligonucleotide-streptavidin conjugates and their application in DNA-directed immobilization (DDI) of proteins.
Curr Protoc Nucleic Acid Chem
; Chapter 12: Unit 12.7, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-18428936
ABSTRACT
Semisynthetic DNA-streptavidin conjugates are synthesized by covalent coupling of thiol-modified DNA oligonucleotides and streptavidin (STV). The resulting conjugates have binding capacities for four equivalents of biotin and a complementary nucleic acid sequence. The conjugates are purified to homogeneity by ultrafiltration and chromatography, and are characterized by spectrophotometry and gel electrophoresis. Subsequently, the conjugates are applied as molecular connectors in the DNA-directed immobilization (DDI) of biotinylated antibodies using DNA microarrays as immobilization matrices. The results are protein microarrays that can be used for the multiplexed detection of various antigens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
DNA
/
Proteínas
/
Estreptavidina
Idioma:
En
Revista:
Curr Protoc Nucleic Acid Chem
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Alemanha
País de publicação:
EEUU
/
ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
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US
/
USA