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MMP19 is essential for T cell development and T cell-mediated cutaneous immune responses.
Beck, Inken M; Rückert, René; Brandt, Katja; Mueller, Markus S; Sadowski, Thorsten; Brauer, Rena; Schirmacher, Peter; Mentlein, Rolf; Sedlacek, Radislav.
Afiliação
  • Beck IM; Institute of Biotechnology, Prague, Czech Republic.
PLoS One ; 3(6): e2343, 2008 Jun 04.
Article em En | MEDLINE | ID: mdl-18523579
Matrix metalloproteinase-19 (MMP19) affects cell proliferation, adhesion, and migration in vitro but its physiological role in vivo is poorly understood. To determine the function of MMP19, we generated mice deficient for MMP19 by disrupting the catalytic domain of mmp19 gene. Although MMP19-deficient mice do not show overt developmental and morphological abnormalities they display a distinct physiological phenotype. In a model of contact hypersensitivity (CHS) MMP19-deficient mice showed impaired T cell-mediated immune reaction that was characterized by limited influx of inflammatory cells, low proliferation of keratinocytes, and reduced number of activated CD8(+) T cells in draining lymph nodes. In the inflamed tissue, the low number of CD8(+) T cells in MMP19-deficient mice correlated with low amounts of proinflammatory cytokines, especially lymphotactin and interferon-inducible T cell alpha chemoattractant (I-TAC). Further analyses showed that T cell populations in the blood of immature, unsensitized mice were diminished and that this alteration originated from an altered maturation of thymocytes. In the thymus, thymocytes exhibited low proliferation rates and the number of CD4(+)CD8(+) double-positive cells was remarkably augmented. Based on the phenotype of MMP19-deficient mice we propose that MMP19 is an important factor in cutaneous immune responses and influences the development of T cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pele / Linfócitos T CD4-Positivos / Linfócitos T CD8-Positivos / Metaloproteinases da Matriz Secretadas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: República Tcheca País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pele / Linfócitos T CD4-Positivos / Linfócitos T CD8-Positivos / Metaloproteinases da Matriz Secretadas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2008 Tipo de documento: Article País de afiliação: República Tcheca País de publicação: Estados Unidos