Mechanisms of copper toxicity in Saccharomyces cerevisiae determined by microarray analysis.
Environ Toxicol
; 23(5): 599-606, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18528910
ABSTRACT
The effect of the heavy metal copper on the expression of a wide spectrum of genes was analyzed by using a DNA microarray. The gene expression profile of baker's yeast Saccharomyces cerevisiae grown in a medium containing a sublethal concentration of cupric sulfate was compared with that of yeast grown in a normal medium. Among approximately 6000 yeast ORFs, 143 ORFs were induced more than twofold to resist copper toxicity after exposure to copper. Copper metallothionein CUP1-1 and CUP1-2 were induced more than 20-fold. Some genes related to sulfur metabolism and oxidative stress response were also up-regulated. This DNA microarray analysis identified several molecular targets of copper toxicity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Regulação Fúngica da Expressão Gênica
/
Sulfato de Cobre
/
Cobre
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Environ Toxicol
Assunto da revista:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Japão