Developmental changes in expression and self-editing of adenosine deaminase type 2 pre-mRNA and mRNA in rat brain and cultured cortical neurons.
Neurosci Res
; 61(4): 398-403, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18534702
ABSTRACT
Adenosine deaminase-1 and -2 (ADAR-1 and -2) are double-stranded RNA-specific enzymes involved in the editing of genes including serotonin 2C receptor (5-HT2CR) mRNA and ADAR-2 pre-mRNA. We reported that the editing efficacy of 5-HT2CR mRNA altered during brain development in rats. The present study aimed to clarify if changes in the expression of ADAR genes and the editing of ADAR-2 pre-mRNA occur during development. The expression level of ADAR-1 mRNA was constant during development, whereas the expression levels of ADAR-2 mRNA and ADAR-2 pre-mRNA markedly increased during development. ADAR-2 pre-mRNA possesses six editing sites. Editing of these sites did not occur during the embryonic period; however, the number of edited sites and the editing frequency at these sites increased after birth and cultivation period. These results suggest that the increases in ADAR-2 pre-mRNA editing and mRNA expression of the enzyme may play a role in development. We also discuss the relationship between 5-HT2CR mRNA editing and the expression/RNA editing of ADAR-1 and ADAR-2 mRNA.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
RNA Mensageiro
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Precursores de RNA
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Adenosina Desaminase
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Edição de RNA
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Regulação da Expressão Gênica no Desenvolvimento
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Neurônios
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Neurosci Res
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Japão