The Symphony protocol for H&E staining of prostatic adenocarcinoma on needle biopsy: a multicentre analysis of 120 cases.
Pathology
; 40(5): 450-6, 2008 Aug.
Article
em En
| MEDLINE
| ID: mdl-18604729
ABSTRACT
AIMS:
The diagnosis of prostate carcinoma is based on a constellation of architectural, nuclear, cytoplasmic, and ancillary features. The aim of this study was to determine if the Ventana Symphony H&E protocol can improve on the detection of prominent nucleoli in foci of prostate cancer.METHODS:
One hundred and twenty cases of Gleason score 3 + 3 = 6 cancers involving 10-30% of one core were retrieved from four academic institutions and two large laboratories (20 cases per institution). The routine H&E and Symphony H&E protocol stained slides from the same case were reviewed centrally in a blinded fashion at the Johns Hopkins Hospital. The mean percentages of prominent nucleoli in malignant and benign glands by visual estimation were recorded for each case. After unblinding, the routine H&E slides were compared in a pair-wise t-test with the corresponding Symphony stained slide.RESULTS:
Within all sites, the Symphony slides showed statistically significantly more prominent nucleoli in the malignant glands than the routine slides.CONCLUSIONS:
The Symphony H&E staining protocol consistently highlights nucleoli in cancer to a greater extent than routine H&E stains and may increase the likelihood of making a diagnosis of limited adenocarcinoma of the prostate in challenging cases.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Coloração e Rotulagem
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Biópsia por Agulha
/
Adenocarcinoma
Tipo de estudo:
Clinical_trials
/
Guideline
Limite:
Humans
/
Male
Idioma:
En
Revista:
Pathology
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos