Reporter genes for real-time in vive monitoring of Ochrobactrum anthropi infection.
FEMS Microbiol Lett
; 286(1): 124-9, 2008 Sep.
Article
em En
| MEDLINE
| ID: mdl-18637041
ABSTRACT
Despite the increasing interest in Ochrobactrum anthropi as an emerging nosocomial pathogen resistant to most commonly used antimicrobials, relatively little is known about the pathogenesis and factors contributing to its virulence. Also, many aspects of interaction between Ochrobactrum spp. and their hosts remain unclear. The ability to monitor O. anthropi infection in the host will facilitate our understanding of the pathogenic mechanisms and will lead to better choices of antimicrobial or additional therapeutic strategies. We have demonstrated the ability to stably express three reporter genes (green fluorescence protein GFP, red fluorescence protein RFP and luciferase Lux) and track the infection in a J774A.1 murine macrophage cell line as well as in the BALB/c mouse. Our results suggest that these reporter genes should improve genetic studies in O. anthropi, particularly those aimed at understanding pathogenesis, virulence factors and host interaction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Bactérias Gram-Negativas
/
Genes Reporter
/
Ochrobactrum anthropi
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Interações Hospedeiro-Patógeno
Limite:
Animals
/
Humans
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos