One-step RT-PCR for detection of Zika virus.
J Clin Virol
; 43(1): 96-101, 2008 Sep.
Article
em En
| MEDLINE
| ID: mdl-18674965
ABSTRACT
BACKGROUND:
Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive.OBJECTIVE:
To develop a one-step RT-PCR assay to detect ZIKV in human serum. STUDYDESIGN:
An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts.RESULTS:
The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected.CONCLUSIONS:
The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas do Envelope Viral
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Zika virus
/
Infecção por Zika virus
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
J Clin Virol
Assunto da revista:
VIROLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Senegal