Changes in pattern of phospholipid acylation during in vivo aging of rat red blood cells.
Am J Physiol
; 261(3 Pt 1): C417-22, 1991 Sep.
Article
em En
| MEDLINE
| ID: mdl-1887869
ABSTRACT
We have investigated the patterns of incorporation of stearic, oleic, and linoleic acids into phosphatidylcholine (PC) and phosphatidylethanolamine (PE) of intact red blood cells of differing age isolated by centrifugation on discontinuous density gradient. Acylation rates of PC and PE elicited marked declines from the reticulocyte to the young erythrocyte stage followed by minimal changes of acylating potency in older cells; this biphasic decay pattern was similar with the three fatty acids. Molar acylation rates were higher for PC than for PE in reticulocytes, whereas they were comparable in erythrocytes. PC served as preferred fatty acid acceptor in circulating red blood cells, a function which was largely accounted for by PC contained in the small percentage of circulating reticulocytes. On a per cell basis, this function of PC was due to the cumulative effects of higher molar acylation rates in reticulocytes and higher content in PC over PE in the red blood cell membrane. Acylation rates in PC and PE increased with the number of unsaturated bonds in the acylating fatty acid, regardless of cell age.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Envelhecimento Eritrocítico
/
Eritrócitos
Limite:
Animals
Idioma:
En
Revista:
Am J Physiol
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
França