Development of a rapid latex enhanced turbidimetric assay for retinol binding protein in urine.
Ann Clin Biochem
; 28 ( Pt 4): 407-11, 1991 Jul.
Article
em En
| MEDLINE
| ID: mdl-1892352
ABSTRACT
We describe a simple, rapid and sensitive homogeneous immunoassay for urinary retinol-binding protein (RBP) using latex particle-enhanced turbidimetric immunoassay. Rabbit anti-human RBP is covalently coupled to 40 nm latex particles and the assay performed on the IL Monarch 2000 centrifugal analyser, with a 20 microL sample volume and the reaction monitored at 340 nm over an 8 min period. The assay range is 0-6 mg/L with a detection limit of 25 micrograms/L. The within and between assay coefficients of variation are less than 1.5% and less than 2.5%, respectively. Comparison with radioimmunoassay for RBP showed good agreement.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação ao Retinol
/
Imunoensaio
/
Nefelometria e Turbidimetria
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Ann Clin Biochem
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Reino Unido
País de publicação:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM