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Hypertonic saline attenuates TNF-alpha-induced NF-kappaB activation in pulmonary epithelial cells.
Nydam, Trevor L; Moore, Ernest E; McIntyre, Robert C; Wright, Franklin L; Gamboni-Robertson, Fabia; Eckels, Phillip C; Banerjee, Anirban.
Afiliação
  • Nydam TL; Center for Trauma Research and Department of Surgery, University of Colorado Health Sciences Center, Denver, Colorado 80045, USA. Trevor.Nydam@UCHSC.edu
Shock ; 31(5): 466-72, 2009 May.
Article em En | MEDLINE | ID: mdl-18948845
ABSTRACT
Resuscitation with hypertonic saline (HTS) attenuates acute lung injury (ALI) and modulates postinjury hyperinflammation. TNF-alpha-stimulated pulmonary epithelium is a major contributor to hemorrhage-induced ALI. We hypothesized that HTS would inhibit TNF-alpha-induced nuclear factor (NF)-kappaB proinflammatory signaling in pulmonary epithelial cells. Therefore, we pretreated human pulmonary epithelial cells (A549) with hypertonic medium (180 mM NaCl) for 30 min, followed by TNF-alpha stimulation (10 ng/mL). Key regulatory steps and protein concentrations in this pathway were assessed for significant alterations. Hypertonic saline significantly reduced TNF-alpha-induced intercellular adhesion molecule 1 levels and NF-kappaB nuclear localization. The mechanism is attenuated phosphorylation and delayed degradation of IkappaB alpha. Hypertonic saline did not alter TNF-alpha-induced p38 mitogen-activated protein kinase phosphorylation or constitutive vascular endothelial growth factor expression, suggesting that the observed inhibition is not a generalized suppression of protein phosphorylation or cellular function. These results show that HTS inhibits TNF-alpha-induced NF-kappaB activation in the pulmonary epithelium and, further, our understanding of its beneficial effects in hemorrhage-induced ALI.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solução Salina Hipertônica / NF-kappa B / Fator de Necrose Tumoral alfa / Células Epiteliais / Pulmão Limite: Humans Idioma: En Revista: Shock Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solução Salina Hipertônica / NF-kappa B / Fator de Necrose Tumoral alfa / Células Epiteliais / Pulmão Limite: Humans Idioma: En Revista: Shock Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Estados Unidos