Transversely illuminated liquid core waveguide based fluorescence detection Fluorometric flow injection determination of aqueous ammonium/ammonia.
Talanta
; 50(3): 617-23, 1999 Oct.
Article
em En
| MEDLINE
| ID: mdl-18967752
ABSTRACT
The analytical performance of a new type of fluorescence detector, based on a transversely illuminated liquid core waveguide (LCW), has been investigated using the determination of NH(3)/NH(4)(+) as the 1-sulfonatoisoindole. With a very inexpensive combination of a miniature Hg blacklight as an excitation source, a colored plastic sheet as the emission filter, and an integrated blue sensitized photodiode-operational amplifier as the detector (totaling <$100 in hardware cost), we were able to achieve a limit of detection (LOD) of 35 nM (1.6 pmol) NH(3) with a linear dynamic range up to 60 muM NH(3). Details of detector construction and performance are given.
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01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Talanta
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS