Isolation and characterization of nitrogen mustard-sensitive mutants of Chinese hamster ovary cells.
Mutat Res
; 254(2): 161-5, 1991 Mar.
Article
em En
| MEDLINE
| ID: mdl-1900571
ABSTRACT
Three nitrogen mustard-sensitive lines of Chinese hamster ovary cells were isolated from mutagenized cultures using the procedure of Thompson et al. (1980). The lines, designated NM1, NM2 and NM3, were 2.1-, 17- and 6.8-fold more sensitive to nitrogen mustard, respectively, than their parent, wild-type, line as determined by the dose required to kill 90% of the cells, IC90. Patterns of cross-sensitivity to other DNA-damaging agents including ultraviolet light, cis-diamminedichloroplatinum, and other alkylating agents were determined for each line. Analysis of these results suggests that the phenotypes of the mutant lines are different from those lines reported previously.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Células Cultivadas
/
Mecloretamina
/
Mutação
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
1991
Tipo de documento:
Article