Culex flavivirus isolates from mosquitoes in Guatemala.
J Med Entomol
; 45(6): 1187-90, 2008 Nov.
Article
em En
| MEDLINE
| ID: mdl-19058647
A new strain of Culex flavivirus (family Flaviviridae, genus Flavivirus, CxFV), an insect virus first described in Japan, was isolated from adult Culex quinquefasciatus Say (Diptera: Culicidae) collected in 2006 from Izabal Department on the Caribbean coast of Guatemala. Mosquito pools were assayed for flavivirus RNA by using flavivirus group-specific primers that amplified a 720-bp region of the nonstructural (NS) 5 gene by standard reverse transcriptase-polymerase chain reaction. From 210 pools (1,699 mosquitoes), eight tested positive, and six of these mosquito pools produced virus isolates in Aedes albopictus Skuse C6/36 cells. Nucleotide sequence comparison of the eight flavivirus RNA-positive pools showed that there was 100% identity among them, and phylogenetic analysis of the NS5 and envelope gene regions indicated that they represent a strain of the recently described CxFV from Japan. This is the first report of an insect flavivirus from Central America.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Culex
/
Flavivirus
Limite:
Animals
País/Região como assunto:
America central
/
Guatemala
Idioma:
En
Revista:
J Med Entomol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Guatemala
País de publicação:
Reino Unido