An NAD-specific glutamate dehydrogenase from cyanobacteria. Identification and properties.
FEBS Lett
; 285(1): 35-8, 1991 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-1906012
ABSTRACT
The unicellular cyanobacterium Synechocystis sp. PCC 6803 presents a hexameric NAD-specific glutamate dehydrogenase with a molecular mass of 295 kDa. The enzyme differs from the NADP-glutamate dehydrogenase found in the same strain and is coded by a different gene. NAD-glutamate dehydrogenase shows a high coenzyme specificity, catalyzes preferentially glutamate formation and presents Km values for ammonium, NADH and 2-oxoglutarate of 4.5 mM, 50 microM and 1.8 mM respectively. An animating role for the enzyme is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cianobactérias
/
Glutamato Desidrogenase
/
Amônia
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Espanha