A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.
Anal Biochem
; 385(2): 383-5, 2009 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-19061853
ABSTRACT
We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Marcadores de Afinidade
/
Proteínas
/
Baculoviridae
/
Clonagem Molecular
/
Vetores Genéticos
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
França