Effect of succinic acid and tween-80 on glucuronidation of 2-ethyl-6-methyl-3-hydroxypyridine.
Bull Exp Biol Med
; 146(1): 56-8, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-19145350
ABSTRACT
We studied the effect of succinic acid on the process of glucuronidation of 2-ethyl-6-methyl-3-hydroxypyridine after peroral and intraperitoneal administration in the form of succinate or a base. Since the basic form of 2-ethyl-6-methyl-3-hydroxypyridine is insoluble in water, it was administered in 5% Tween-80. It was necessary to evaluate also the effect of Tween-80 on glucuronidation of 2-ethyl-6-methyl-3-hydroxypyridine in different administration routes. Quantitative assay of glucuronidated fractions was performed by the method of reversed-phase HPLC with fluorometrical detection. The detection limit for this method was 10 ng/ml. We confirmed that the major excretion pathway for 2-ethyl-6-methyl-3-hydroxypyridine is conjugation with glucuronic acid. It was found that succinic acid increased excretion of glucuronidated metabolite after both peroral and intraperitoneal administration of 2-ethyl-6-methyl-3-hydroxypyridine in the form of succinate and base in 5% Tween-80. The effect of Tween-80 was detected only after peroral administration, which was probably related to its effect on absorption of this compound. Tween-80 increased excretion of glucuronate after peroral administration of 2-ethyl-6-methyl-3-hydroxypyridine in the form of succinate and in 5% Tween solution.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Picolinas
/
Polissorbatos
/
Tensoativos
/
Ácido Succínico
/
Ácido Glucurônico
/
Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Bull Exp Biol Med
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Federação Russa