Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates.
Science
; 323(5919): 1350-3, 2009 Mar 06.
Article
em En
| MEDLINE
| ID: mdl-19150808
ABSTRACT
Successful cell division requires that chromosomes attach to opposite poles of the mitotic spindle (bi-orientation). Aurora B kinase regulates chromosome-spindle attachments by phosphorylating kinetochore substrates that bind microtubules. Centromere tension stabilizes bi-oriented attachments, but how physical forces are translated into signaling at individual centromeres is unknown. Using fluorescence resonance energy transfer-based biosensors to measure localized phosphorylation dynamics in living cells, we found that phosphorylation of an Aurora B substrate at the kinetochore depended on its distance from the kinase at the inner centromere. Furthermore, repositioning Aurora B closer to the kinetochore prevented stabilization of bi-oriented attachments and activated the spindle checkpoint. Thus, centromere tension can be sensed by increased spatial separation of Aurora B from kinetochore substrates, which reduces phosphorylation and stabilizes kinetochore microtubules.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Centrômero
/
Cromossomos Humanos
/
Proteínas Serina-Treonina Quinases
/
Cinetocoros
/
Microtúbulos
/
Fuso Acromático
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos