[Molecular subtyping of Staphylococcus aureus isolated from a severe food-poisoning].
Zhonghua Yu Fang Yi Xue Za Zhi
; 42(9): 672-6, 2008 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-19175179
ABSTRACT
OBJECTIVE:
To study the molecular types of Staphylococcus aureus isolated from a severe food-poisoning and to trace the possible strains.METHODS:
Real-time PCR was applied to detect nuc gene as a specific marker for S. aureus, mecA gene encoding methicillin resistance and 5 other genes encoding staphylococcal enterotoxins (sea, seb, see, sed, see). Isolates were also performed with 16S rRNA oligonucleotide sequence analyzing by DNAStar MegAlign 5.0 software and pulse-field gel electrophoresis (PFGE) by BioNumerics Version 4.0 software.RESULTS:
The nuc gene was detected from the 10 isolated strains, sea and seb genes were detected from 7 strains. There were 4 16 S rRNA types and 5 PFGE types found from all the strains.CONCLUSIONS:
Three relative S. aureus strains were involved in the severe food-poisoning at least. Molecular subtyping might give a molecular epidemiological evidence and support the source tracing of an outbreak.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Intoxicação Alimentar Estafilocócica
/
Staphylococcus aureus
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhonghua Yu Fang Yi Xue Za Zhi
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China