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Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule.
Varich, Natalia L; Kochergin-Nikitsky, Konstantin S; Usachev, Evgeny V; Usacheva, Olga V; Prilipov, Alexei G; Webster, Robert G; Kaverin, Nikolai V.
Afiliação
  • Varich NL; D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
  • Kochergin-Nikitsky KS; D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
  • Usachev EV; D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
  • Usacheva OV; D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
  • Prilipov AG; D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098 Moscow, Russia.
  • Webster RG; Department of Pathology, University of Tennessee, Memphis, TN 38105, USA.
  • Kaverin NV; Division of Virology, Department of Infectious Diseases, St Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105 3678, USA.
J Gen Virol ; 90(Pt 7): 1730-1733, 2009 Jul.
Article em En | MEDLINE | ID: mdl-19297605
The locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas do Core Viral / Proteínas de Ligação a RNA / Epitopos de Linfócito B / Anticorpos Monoclonais / Anticorpos Antivirais Limite: Animals Idioma: En Revista: J Gen Virol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas do Core Viral / Proteínas de Ligação a RNA / Epitopos de Linfócito B / Anticorpos Monoclonais / Anticorpos Antivirais Limite: Animals Idioma: En Revista: J Gen Virol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Reino Unido