A novel short hairpin RNA (shRNA) expression system promotes Sox9-dependent gene silencing.
Plasmid
; 62(1): 50-5, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-19389425
ABSTRACT
Cartilage development and function are dependent on a temporally integrated program of gene expression. With the advent of RNA interference (RNAi), artificial control of these complex programs becomes a possibility, limited only by the ability to regulate and express the RNAi. Using existing methods for production of RNAi's, we have constructed a plasmid-based short hairpin RNA (shRNA) expression system under control of the human pol III H1 promoter and supplemented this promoter with DNA binding sites for the cartilage-specific transcription factor Sox9. The resulting shRNA expression system displays robust, Sox9-dependent gene silencing. Dependence on Sox9 expression was confirmed by electrophoretic mobility shift assays. The ability of the system to regulate heterologously expressed Sox9 was demonstrated by Western blot, as a function of both Sox9 to shRNA ratio, as well as time from transfection. This novel expression system supports auto-regulatory gene silencing, providing a tissue-specific feedback mechanism for temporal control of gene expression. Its applications for both basic mechanistic studies and therapeutic purposes should facilitate the design and implementation of innovative tissue engineering strategies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Genéticas
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Inativação Gênica
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RNA Interferente Pequeno
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Fatores de Transcrição SOX9
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Conformação de Ácido Nucleico
Limite:
Humans
Idioma:
En
Revista:
Plasmid
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos