CD46 expression and HHV-6 infection in patients with multiple sclerosis.
Acta Neurol Scand
; 120(4): 246-50, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-19456309
ABSTRACT
OBJECTIVES:
The aim of this study was to investigate the possible association between the levels of the CD46 expression, and the presence and viral load of HHV-6 in patients with multiple sclerosis (MS).METHODS:
We collected blood and serum samples of 103 patients with MS and the same number of healthy blood donors (HBD); total DNA and RNA was extracted from peripheral blood mononuclear cells (PBMCs) and serum, and then analyzed using quantitative real-time PCR for the detection of CD46 transcripts and HHV-6 genomes; the expression of rRNA18s was used for the calculation of the relative expression of CD46.RESULTS:
Almost 80% of patients with MS had increased levels of CD46 in comparison with HBD, and a positive correlation was also found between the over-expression of CD46 in patients with MS and the HHV-6 DNA prevalence and viral load in the blood and serum.DISCUSSION:
Therefore, the up-regulation of CD46 expression in patients with MS with HHV-6 infection could constitute an immunopathogenic factor that should be further investigated to elucidate its role in MS.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Herpesvirus Humano 6
/
Infecções por Roseolovirus
/
Proteína Cofatora de Membrana
/
Esclerose Múltipla
Tipo de estudo:
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Acta Neurol Scand
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Espanha