Prolonged seizure activity leads to increased Protein Kinase A activation in the rat pilocarpine model of status epilepticus.
Brain Res
; 1283: 167-76, 2009 Aug 04.
Article
em En
| MEDLINE
| ID: mdl-19501060
ABSTRACT
Status epilepticus is a life-threatening form of seizure activity that represents a major medical emergency associated with significant morbidity and mortality. Protein Kinase A is an important regulator of synaptic strength that may play an important role in the development of status epilepticus-induced neuronal pathology. This study demonstrated an increase in PKA activity against exogenous and endogenous substrates during later stages of SE. As SE progressed, a significant increase in PKA-mediated phosphorylation of an exogenous peptide substrate was demonstrated in cortical structures. The increased activity was not due to altered expression of either regulatory or catalytic subunits of the enzyme. Through the use of phospho-specific antibodies, this study also investigated the effects of SE on the phosphorylation of the GluR1 subunit of the AMPA subtype of glutamate receptor. After the onset of continuous seizure activity, an increase in phosphorylation of the PKA site on the GluR1 subunit of the AMPA receptor was observed. These data suggest a potential mechanism by which SE may increase neuronal excitability in the cortex, potentially leading to maintenance of seizure activity or long-term neuronal pathology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estado Epiléptico
/
Córtex Cerebral
/
Proteínas Quinases Dependentes de AMP Cíclico
/
Epilepsia
Limite:
Animals
Idioma:
En
Revista:
Brain Res
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos