Isolation and characterization of a novel lysine racemase from a soil metagenomic library.
Appl Environ Microbiol
; 75(15): 5161-6, 2009 Aug.
Article
em En
| MEDLINE
| ID: mdl-19502445
ABSTRACT
A lysine racemase (lyr) gene was isolated from a soil metagenome by functional complementation for the first time by using Escherichia coli BCRC 51734 cells as the host and d-lysine as the selection agent. The lyr gene consisted of a 1,182-bp nucleotide sequence encoding a protein of 393 amino acids with a molecular mass of about 42.7 kDa. The enzyme exhibited higher specific activity toward lysine in the l-lysine-to-d-lysine direction than in the reverse reaction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
Biblioteca Gênica
/
Isomerases de Aminoácido
/
Lisina
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Taiwan