Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells.
Cytotherapy
; 11(4): 443-7, 2009.
Article
em En
| MEDLINE
| ID: mdl-19513899
ABSTRACT
BACKGROUND AIMS:
A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs.METHODS:
Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. RESULTS ANDCONCLUSIONS:
Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Células Estromais
/
Sangue Fetal
/
Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
En
Revista:
Cytotherapy
Assunto da revista:
TERAPEUTICA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Canadá