Compact quantum dot probes for rapid and sensitive DNA detection using highly efficient fluorescence resonant energy transfer.
Nanotechnology
; 20(30): 305502, 2009 Jul 29.
Article
em En
| MEDLINE
| ID: mdl-19581695
ABSTRACT
We developed a simple method for quickly synthesizing compact quantum dot (QD)-DNA probes for sensitive DNA detection using fluorescence resonant energy transfer (FRET). The density of DNA probes on the QD surface was controlled to avoid steric hindrance and to promote rapid hybridization with target DNA molecules. The radius of the final QDs was only around 3 nm after applying the functional coating, enabling highly efficient energy transfer. It was demonstrated that nearly 70% transfer efficiency could be achieved with only a few DNA molecules on each QD and that the FRET-based DNA detection could be carried out within 10 min with a sub-nM detection limit. Theoretical analysis was also performed to confirm our results.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Sondas de DNA
/
Transferência Ressonante de Energia de Fluorescência
/
Pontos Quânticos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Nanotechnology
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos